HPLC, Oligonucleotides up to chain lengths of 40 bases
Storage temperature
RT
Scope of delivery
1x semi-preparative HPLC column, instruction leaflet, certificate of analysis
Hazardous material
No
Method
HPLC, preparative
Brand
NUCLEOGEN
Base material
Silica gel
Phase
NUCLEOGEN 60-7 DEAE
USP listing
Not available or proprietary
Surface chemistry
Silica gel based anion exchanger with hydrophilic coating
Mode
Ion exchange (IEX), Special application or diverse
Type
VP HPLC column (preparative)
Hardware
Stainless steel
Column length
125 mm
Column inner diameter
10 mm
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Particle size
7 µm
Eluent in HPLC column
Methanol
Endcapped
No
Carbon content
Not available or proprietary
pH stability
2.5–8.0
Pore size
60 Å
Specific surface according to BET
Not available or proprietary
Temperature stability (max)
70 °C
Pressure stability (max)
250 bar
DESCRIPTION
Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For separation of oligonucleotides up to chain lengths of 40 bases with recoveries > 95 %. Capacity 200 A260/mL (~ 300 A260 for a 125 x 4 mm ID column, 1875 A260 for a 125 x 10 mm ID column). Preparative separations possible when using higher flow rates and longer gradient times.
+852-3069 6950
css@gugent.com.hk
