MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN730299	SPE columns, CHROMABOND C18 Hydra, 45 µm, 6 mL/500 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 Hydra Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18, RP18) modified silica phase, not endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 500 mg Column volume/adsorbent weight 6 mL/500 mg Recommended application(s) Especially for polar compounds, Nitroaromatics, Pesticides and their polar degradation products, Pharmaceuticals, Phenols, Phenoxycarboxylic acids Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730300	SPE columns, CHROMABOND C18 Hydra, 45 µm, 6 mL/1000 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 Hydra Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18, RP18) modified silica phase, not endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 1000 mg Column volume/adsorbent weight 6 mL/1000 mg Recommended application(s) Especially for polar compounds, Nitroaromatics, Pesticides and their polar degradation products, Pharmaceuticals, Phenols, Phenoxycarboxylic acids Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730301	SPE columns, CHROMABOND C18 Hydra, 45 µm, 6 mL/2000 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase C18 Hydra Mode Reversed phase (RP) Base material Silica gel Surface chemistry Octadecyl (ODS, C18, RP18) modified silica phase, not endcapped Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 2000 mg Column volume/adsorbent weight 6 mL/2000 mg Recommended application(s) Especially for polar compounds, Nitroaromatics, Pesticides and their polar degradation products, Pharmaceuticals, Phenols, Phenoxycarboxylic acids Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Irregular Specific surface according to BET 500 m²/g pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730109	SPE columns, CHROMABOND NAN, 45 µm, 3 mL/400/1400/400 mg	Special offer	Add to Cart
Hazardous material Yes Method Solid phase extraction (SPE) Brand CHROMABOND Phase NAN Mode Special application or diverse Base material Sodium sulfate/silica gel Surface chemistry Special combination phase, N- sodium sulfate (NA₂SO₄), A- silica gel/silver nitrate (SiOH/AgNO₃) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 3 mL Filling quantity 400/1400/400 mg Column volume/adsorbent weight 3 mL/400/1400/400 mg Recommended application(s) Extraction of PCBs from sludge Particle type Fully porous particles (FPP) Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730149	SPE columns, CHROMABOND NAN, 45 µm, 6 mL/700/2000/700 mg	Special offer	Add to Cart
Hazardous material Yes Method Solid phase extraction (SPE) Brand CHROMABOND Phase NAN Mode Special application or diverse Base material Sodium sulfate/silica gel Surface chemistry Special combination phase, N- sodium sulfate (NA₂SO₄), A- silica gel/silver nitrate (SiOH/AgNO₃) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 700/2000/700 mg Column volume/adsorbent weight 6 mL/700/2000/700 mg Selling unit 30 columns, 250 columns Recommended application(s) Extraction of PCBs from sludge Particle type Fully porous particles (FPP) Particle shape Irregular pH stability 2.0–8.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730149G	SPE glass columns, CHROMABOND NAN, 45 µm, 6 mL/700/2000/700 mg	Special offer	Add to Cart
Hazardous material Yes Method Solid phase extraction (SPE) Brand CHROMABOND Phase NAN Mode Special application or diverse Base material Sodium sulfate/silica gel Surface chemistry Special combination phase, N- sodium sulfate (NA₂SO₄), A- silica gel/silver nitrate (SiOH/AgNO₃) Column type SPE glass column Hardware Glass columns (borosilicate type I) with glass fiber (GF) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 700/2000/700 mg Column volume/adsorbent weight 6 mL/700/2000/700 mg Recommended application(s) Extraction of PCBs from sludge Particle type Fully porous particles (FPP) Particle shape Irregular pH stability 2.0–8.0 Filter element material Glass fiber (GF) Storage temperature RT
	MN730283	SPE columns, CHROMABOND PFAS, 6 mL/300 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase PFAS Mode Reversed phase (RP) Base material Polymer-based Surface chemistry Special combination phase with weak anion exchanger Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 300 mg Column volume/adsorbent weight 6 mL/300 mg Selling unit 30 columns, 250 columns Recommended application(s) Per- and polyfluoroalkyl substances (PFAS) from water, PFAS from sediments (contaminated soils), PFAS from textiles Particle type Fully porous particles(FPP) Particle size Not available or proprietary Particle shape Spherical Specific surface according to BET Not available or proprietary pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN7300009	SPE columns, CHROMABOND PFAS, 3 mL/120 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase PFAS Mode Reversed phase (RP) Base material Polymer-based Surface chemistry Special combination phase with weak anion exchanger Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 3 mL Filling quantity 120 mg Column volume/adsorbent weight 3 mL/120 mg Selling unit 30 columns, 250 columns Recommended application(s) Per- and polyfluoroalkyl substances (PFAS) from water, PFAS from sediments (contaminated soils), PFAS from textiles Particle type Fully porous particles(FPP) Particle size Not available or proprietary Particle shape Spherical Specific surface according to BET Not available or proprietary pH stability 1.0–14.0 Filter element material Polyethylene (PE)
	MN730165	SPE columns, CHROMABOND Carbon A, 6 mL/500 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase Carbon A Mode Special application or diverse Base material Activated carbon Surface chemistry Activated carbon, highly porous Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 500 mg Column volume/adsorbent weight 6 mL/500 mg Recommended application(s) Acrylamide from water (DIN 38413-6) Particle type Fully porous particles (FPP) Particle size Not available or proprietary Particle shape Spherical Specific surface according to BET > 1000 m²/g pH stability Not available or not applicable Filter element material Polyethylene (PE) Storage temperature RT
	MN730167	SPE columns, CHROMABOND Carbon A, 6 mL/1 g	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase Carbon A Mode Special application or diverse Base material Activated carbon Surface chemistry Activated carbon, highly porous Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 1000 mg Column volume/adsorbent weight 6 mL/1 g Recommended application(s) Acrylamide from water (DIN 38413-6) Particle type Fully porous particles (FPP) Particle size Not available or proprietary Particle shape Spherical Specific surface according to BET > 1000 m²/g pH stability Not available or not applicable Filter element material Polyethylene (PE) Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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