MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN730956P45	SPE columns, CHROMABOND HR-XC, 45 µm, 3 mL/60 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XC Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 3 mL Filling quantity 60 mg Column volume/adsorbent weight 3 mL/60 mg Recommended application(s) Amines, Bases with pKa 2–10, Basic active ingredients from strongly matrix-contaminated samples, Basic analytes, Fungicides from food Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Spherical Specific surface according to BET 800 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730969P45	SPE columns, CHROMABOND HR-XC, 45 µm, 1 mL/30 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XC Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 1 mL Filling quantity 30 mg Column volume/adsorbent weight 1 mL/30 mg Recommended application(s) Amines, Bases with pKa 2–10, Basic active ingredients from strongly matrix-contaminated samples, Basic analytes, Fungicides from food Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Spherical Specific surface according to BET 800 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730731	SPE columns, CHROMABOND HR-XCW, 85 µm, 1 mL/30 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XCW Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 1 mL Filling quantity 30 mg Column volume/adsorbent weight 1 mL/30 mg Recommended application(s) Basic active ingredients from strongly matrix-contaminated samples, Basic compounds, Quaternary amines, Strong bases with pKa > 10 Particle type Fully porous particles (FPP) Particle size 85 µm Particle shape Spherical Specific surface according to BET 850 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730733	SPE columns, CHROMABOND HR-XCW, 85 µm, 1 mL/100 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XCW Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 1 mL Filling quantity 100 mg Column volume/adsorbent weight 1 mL/100 mg Recommended application(s) Basic active ingredients from strongly matrix-contaminated samples, Basic compounds, Quaternary amines, Strong bases with pKa > 10 Particle type Fully porous particles (FPP) Particle size 85 µm Particle shape Spherical Specific surface according to BET 850 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730735	SPE columns, CHROMABOND HR-XCW, 85 µm, 3 mL/60 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XCW Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 3 mL Filling quantity 60 mg Column volume/adsorbent weight 3 mL/60 mg Recommended application(s) Basic active ingredients from strongly matrix-contaminated samples, Basic compounds, Quaternary amines, Strong bases with pKa > 10 Particle type Fully porous particles (FPP) Particle size 85 µm Particle shape Spherical Specific surface according to BET 850 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730737	SPE columns, CHROMABOND HR-XCW, 85 µm, 6 mL/150 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XCW Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 150 mg Column volume/adsorbent weight 6 mL/150 mg Recommended application(s) Basic active ingredients from strongly matrix-contaminated samples, Basic compounds, Quaternary amines, Strong bases with pKa > 10 Particle type Fully porous particles (FPP) Particle size 85 µm Particle shape Spherical Specific surface according to BET 850 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730739	SPE columns, CHROMABOND HR-XCW, 85 µm, 3 mL/200 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XCW Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 3 mL Filling quantity 200 mg Column volume/adsorbent weight 3 mL/200 mg Recommended application(s) Basic active ingredients from strongly matrix-contaminated samples, Basic compounds, Quaternary amines, Strong bases with pKa > 10 Particle type Fully porous particles (FPP) Particle size 85 µm Particle shape Spherical Specific surface according to BET 850 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730741	SPE columns, CHROMABOND HR-XCW, 85 µm, 3 mL/500 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XCW Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 3 mL Filling quantity 500 mg Column volume/adsorbent weight 3 mL/500 mg Recommended application(s) Basic active ingredients from strongly matrix-contaminated samples, Basic compounds, Quaternary amines, Strong bases with pKa > 10 Particle type Fully porous particles (FPP) Particle size 85 µm Particle shape Spherical Specific surface according to BET 850 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730743	SPE columns, CHROMABOND HR-XCW, 85 µm, 6 mL/500 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XCW Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 6 mL Filling quantity 500 mg Column volume/adsorbent weight 6 mL/500 mg Recommended application(s) Basic active ingredients from strongly matrix-contaminated samples, Basic compounds, Quaternary amines, Strong bases with pKa > 10 Particle type Fully porous particles (FPP) Particle size 85 µm Particle shape Spherical Specific surface according to BET 850 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT
	MN730731P45	SPE columns, CHROMABOND HR-XCW, 45 µm, 1 mL/30 mg	Special offer	Add to Cart
Hazardous material No Method Solid phase extraction (SPE) Brand CHROMABOND Phase HR-XCW Mode Mixed mode (RP and Ion exchange (IEX)) Base material PS/DVB copolymer Surface chemistry Hydrophobic spherical polystyrene-divinylbenzene copolymer (PS/DVB) Column type SPE column Hardware Polypropylene (PP) columns with polyethylene (PE) filter elements Column shape Open tubular syringe-shaped column with Luer tip outlet Column volume 1 mL Filling quantity 30 mg Column volume/adsorbent weight 1 mL/30 mg Recommended application(s) Basic active ingredients from strongly matrix-contaminated samples, Basic compounds, Quaternary amines, Strong bases with pKa > 10 Particle type Fully porous particles (FPP) Particle size 45 µm Particle shape Spherical Specific surface according to BET 850 m²/g pH stability 1.0–14.0 Filter element material Polyethylene (PE) Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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