Merck offers a wide range of very successful and innovative packing materials for HPLC, manufactured in the unique and easy-to-use LiChroCART® cartridge system with its manu-CART® holder. In addition to proven and successful packing materials such as LiChrosorb®, LiChrospher® and Superspher® (irregular, regular and perfectly spherical silica gel sorbents), special sorbents such as Aluspher® (Al2O3) and several chiral phases such as the highly pure Purospher® and Purospher® STAR stationary phases are also available, and they ensure highest performance and efficient chromatography today and tomorrow.
LiChrospher® RP-18 is a reliable and versatile traditionally produced spherical silica gel carrier with C-18 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a Hibar® 250-4 HPLC column. For mounting of a LiChroCART® 4-4 guard column, the guard column holder 1.51487 has to be used.
LiChrosorb® Si 60 is a reliable irregular shaped silica sorbents with a pore diameter of 60A for normal phase chromatography. This sorbent is packed into a Hibar® 250-4 HPLC column. For mounting of a LiChroCART® 4-4 guard column, the guard column holder 1.51487 has to be used.
Purospher® STAR RP-18 endcapped is the best choice for HPLC separation of basic, neutral, metal chelating samples or indeed any other format. Its outstanding performance and stability, allows a maximum flexibility in method development. Robust methods can be developed across the entire pH spectrum from 1.5 to 10.5 enabling the use of the complete range of mobile phases.
Chromolith® CapRod is a capillary column that combines the speed of monolithic silica technology with the sensitivity of nano-LC, hence enabling new productivity levels for high-throughput proteomics LC applications. In contrast to microparticulate columns Chromolith® CapRod can be operated at significantly higher flow rates without loss of performance. Separations can be achieved at 1-3 µL/min compared to 200-400 nL/min for microparticulate columns.
Chromolith® CapRod is a capillary column that combines the speed of monolithic silica technology with the sensitivity of nano-LC, hence enabling new productivity levels for high-throughput proteomics LC applications. In contrast to microparticulate columns Chromolith® CapRod can be operated at significantly higher flow rates without loss of performance. Separations can be achieved at 1-3 µL/min compared to 200-400 nL/min for microparticulate columns.
Chromolith® CapRod is a capillary column that combines the speed of monolithic silica technology with the sensitivity of nano-LC, hence enabling new productivity levels for high-throughput proteomics LC applications. In contrast to microparticulate columns Chromolith® CapRod can be operated at significantly higher flow rates without loss of performance. Separations can be achieved at 1-3 µL/min compared to 200-400 nL/min for microparticulate columns.
Chromolith® HighResolution CapRod capillary columns have significantly increased efficiency and give better resolution of complex samples, such as peptide mixtures. In order to achieve this higher resolution, the macropore of the monolithic silica has been reduced in size. Typical flow rates are in the region 0.1 - 0.5 µL/min.
Chromolith® CapRod is a capillary column that combines the speed of monolithic silica technology with the sensitivity of nano-LC, hence enabling new productivity levels for high-throughput proteomics LC applications. In contrast to microparticulate columns Chromolith® CapRod can be operated at significantly higher flow rates without loss of performance. Separations can be achieved at 1-3 µL/min compared to 200-400 nL/min for microparticulate columns.
Chromolith® HighResolution CapRod capillary columns have significantly increased efficiency and give better resolution of complex samples, such as peptide mixtures. In order to achieve this higher resolution, the macropore of the monolithic silica has been reduced in size. Typical flow rates are in the region 0.1 - 0.5 µL/min.
This monolithic Trapping Column is a capillary trap that is used to concentrate samples before transfering them to the analytical Chromolith® CapRod column. Typical flow rates are above 1 µL/min.
LiChrosorb® – A Successful Packing Material From the Start!
Merck's LiChrosorb® is one of the most successful and reliable packing materials used in HPLC. LiChrosorb® packing materials offer the complete program of non-polar derivatives (RP-8, RP-18, RP-select B), polar derivatives (Si 60 and Si 100) and medium-polar derivatives (NH2, CN and DIOL). In addition to the analytical cartridges and columns such as LiChroCART® 250-4 and Hibar® RT 250-4, Merck offers LiChroCART® 250-10 semi-preparative cartridges as well as Hibar® RT 250-10 ready-to-use columns, packed with different LiChrosorb® packing materials.
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