Cytiva - LC Columns - Chromatography - Lab supplies

Streamline your chromatography workflow with prepacked columns that come ready to use with chromatography resins.

Catalog No.		Product	Net Price
	CYT29036230	Superdex 75 3.2/300	Special offer	Add to Cart
Bed volume 2.4 Flow rate 0.01 ml/min-0.10 ml/min Pressure max. (over the packed bed during operation) 24 bar [2.4 MPa] (348 psi) Pack size 1 Bed height 300 mm Order Information Not suitable for use with ÄKTAprime plus system Column i.d. 3.2 Sample volume Up to 50 µl Storage 4 to 30°C, 20% Ethanol Bed dimensions 3.2 × 300 mm
	CYT29148722	Superdex™ 75 Increase 5/150 GL	2989.5	Add to Cart
Bed volume ~ 24 mL Bed height 300 to 310 mm Bed support Filter Material (bed support) Polyethylene (PE) filter Material (column tube) Borosilicate glass Column i.d. 10 Chemical stability Conditions where the resin may be operated without significant change in function: • All commonly used aqueous buffers, pH 3 to 12 • Urea, up to 8 M • Ionic and nonionic detergents, e.g., 1% SDS • Guanidine hydrochloride, up to 6 M • Acetic acid, up to 1 M • Methanol, up to 10% • Isopropanol, up to 5% • Dithiothreitol (DTT), up to 5 mM • Sodium hydroxide, up to 0.5 M Conditions where the resin can be subjected to cleaning- or sanitization-in-place without significant change in function: • Acetonitrile, up to 30% • Sodium hydroxide, up to 1 M • Ethanol, up to 70% • Methanol, up to 70% • Isopropanol, up to 30% • Hydrochloric acid, up to 0.1 M • Trifluoroacetic acid, up to 1% • Formic acid, up to 70% Avoid: • Oxidizing agents • Unfiltered samples and eluents Sample volume 25 to 500 µL Bed dimensions 10 × 300 mm Matrix Composite of cross-linked agarose and dextran pH stability, operational 3 to 12 Theoretical plates > 43 000 m^-1
	CYT29148721	Superdex™ 75 Increase 10/300 GL	3012	Add to Cart
Bed volume ~ 24 mL Bed height 300 to 310 mm Bed support Filter Material (bed support) Polyethylene (PE) filter Material (column tube) Borosilicate glass Column i.d. 10 Chemical stability Conditions where the resin may be operated without significant change in function: • All commonly used aqueous buffers, pH 3 to 12 • Urea, up to 8 M • Ionic and nonionic detergents, e.g., 1% SDS • Guanidine hydrochloride, up to 6 M • Acetic acid, up to 1 M • Methanol, up to 10% • Isopropanol, up to 5% • Dithiothreitol (DTT), up to 5 mM • Sodium hydroxide, up to 0.5 M Conditions where the resin can be subjected to cleaning- or sanitization-in-place without significant change in function: • Acetonitrile, up to 30% • Sodium hydroxide, up to 1 M • Ethanol, up to 70% • Methanol, up to 70% • Isopropanol, up to 30% • Hydrochloric acid, up to 0.1 M • Trifluoroacetic acid, up to 1% • Formic acid, up to 70% Avoid: • Oxidizing agents • Unfiltered samples and eluents Sample volume 25 to 500 µL Bed dimensions 10 × 300 mm Matrix Composite of cross-linked agarose and dextran pH stability, operational 3 to 12 Theoretical plates > 43 000 m^-1
	CYT29148723	Superdex™ 75 Increase 3.2/300	3612	Add to Cart
Bed volume ~ 24 mL Bed height 300 to 310 mm Bed support Filter Material (bed support) Polyethylene (PE) filter Material (column tube) Borosilicate glass Column i.d. 10 Chemical stability Conditions where the resin may be operated without significant change in function: • All commonly used aqueous buffers, pH 3 to 12 • Urea, up to 8 M • Ionic and nonionic detergents, e.g., 1% SDS • Guanidine hydrochloride, up to 6 M • Acetic acid, up to 1 M • Methanol, up to 10% • Isopropanol, up to 5% • Dithiothreitol (DTT), up to 5 mM • Sodium hydroxide, up to 0.5 M Conditions where the resin can be subjected to cleaning- or sanitization-in-place without significant change in function: • Acetonitrile, up to 30% • Sodium hydroxide, up to 1 M • Ethanol, up to 70% • Methanol, up to 70% • Isopropanol, up to 30% • Hydrochloric acid, up to 0.1 M • Trifluoroacetic acid, up to 1% • Formic acid, up to 70% Avoid: • Oxidizing agents • Unfiltered samples and eluents Sample volume 25 to 500 µL Bed dimensions 10 × 300 mm Matrix Composite of cross-linked agarose and dextran pH stability, operational 3 to 12 Theoretical plates > 43 000 m^-1
	CYT29036225	Superose 12 3.2/300	Special offer	Add to Cart
Bed volume 2.4 Flow rate 0.01 ml/min-0.10 ml/min Pressure max. (over the packed bed during operation) 24 bar [2.4MPa] (350 psi) Bed height 300 mm Order Information Not suitable for use with ÄKTAprime plus system Column i.d. 3.2 Sample volume 4 - 50 µl Storage 4 to 30°C Bed dimensions 3.2 × 300 mm Theoretical plates > 40000 m-1
	CYT28950515	HiScreen SP HP	190.5	Add to Cart
Bed volume 4.7 Pressure max. (over the packed bed during operation) 3 bar [0.3 MPa] (44 psi) Pack size 1 × 4.7 mL Bed height 100 mm Column i.d. 7.7 Storage 4 to 30ºC, 20% Ethanol, 0.2 M Sodium Acetate Bed dimensions 7.7 × 100 mm Flow velocity < 150 cm/h
	CYT28936551	HisPrep FF 16/10	699	Add to Cart
Pack size 20 mL
	CYT29048586	HisTrap excel, 1 x 1 ml	87	Add to Cart
Chromatography technique Histidine-tagged protein purification Bed volume 1 Flow rate 1 ml/min - 4 ml/min Pressure max. (over the packed bed during operation) 5 bar [0.5 MPa] (70 psi) Pack size 5 × 1 mL Bed height 25 mm Column i.d. 7 Binding Capacity/Column At least 10 mg histidine-tagged protein/ml sedimented medium. Storage 4 to 30°C, 20% ethanol Bed dimensions 7 × 25 mm
	CYT17371205	HisTrap excel, 5 x 1 ml	340.5	Add to Cart
Chromatography technique Histidine-tagged protein purification Bed volume 1 Flow rate 1 ml/min - 4 ml/min Pressure max. (over the packed bed during operation) 5 bar [0.5 MPa] (70 psi) Pack size 5 × 1 mL Bed height 25 mm Column i.d. 7 Binding Capacity/Column At least 10 mg histidine-tagged protein/ml sedimented medium. Storage 4 to 30°C, 20% ethanol Bed dimensions 7 × 25 mm
	CYT17371206	HisTrap excel, 5 x 5 ml	1111.5	Add to Cart
Chromatography technique Histidine-tagged protein purification Bed volume 1 Flow rate 1 ml/min - 4 ml/min Pressure max. (over the packed bed during operation) 5 bar [0.5 MPa] (70 psi) Pack size 5 × 1 mL Bed height 25 mm Column i.d. 7 Binding Capacity/Column At least 10 mg histidine-tagged protein/ml sedimented medium. Storage 4 to 30°C, 20% ethanol Bed dimensions 7 × 25 mm

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Overview

Affinity antibody

Prepacked protein A columns, protein G columns, and other antibody affinity purification products

Affinity specific groups

Affinity chromatography products for purification of proteins or other biomolecules from complex mixtures.

Affinity tagged protein

Purification of tagged proteins including fusions with GST, histidine, Strep-tag™ II, and MBP

Desalting and buffer exchange

Desalting columns remove salts from biological samples prior to further purification or end use.

Hydrophobic interaction

Use prepacked HIC chromatography columns for protein capture, intermediate purification, or polishing steps.

Immunoprecipitation

Use immunoprecipitation columns to isolate small amounts of target proteins

Ion exchange

Use ion exchange columns to enhance your biomolecule purification processes

Multimodal columns

Use multimodal or mixed mode chromatography for increased selectivity, high salt conditions, or process efficiency.

Reversed phase

Reverse phase chromatography columns for high-resolution separations

Size exclusion

Use size exclusion columns to separate proteins and other biomolecules based on size and shape.

Resources

HiScreen™ MabSelect™ VL Prepacked columns Instructions for Use

Guide to bioprocess chromatography resins, membranes, and fibers

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