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Gas chromatography (GC) is a chromatographic technique used for the rapid separation of volatile compounds using an inert carrier gas (mobile phase). The mobile phase drives the gaseous compounds through the heated column which is coated with a solid or liquid stationary phase. The differences in the chemical and physical properties of the vaporized compounds and their interactions with the stationary phase are the basis of the separation process.
Packed GC Columns
Capillary Chiral Columns
Capillary Extremely Polar Columns
Capillary Highly Polar Columns
Capillary Intermediate Polar Columns
Capillary Non-Polar Columns
Capillary PLOT Columns
Capillary Polar Columns
Packed GC Columns
Packed GC columns consist of stainless steel, PTFE, or glass tubing filled with packing material (stationary phase). Packed columns can handle larger sample volumes and are not susceptible to contamination. Glass columns are used for applications requiring high inertness, metal columns are utilized for less demanding applications, and PTFE columns are preferred for low temperature applications.
Capillary GC Columns
Capillary GC columns are best suited for highly sensitive assays. Capillary columns consist of a thin, fused silica glass tube in which the stationary phase is chemically bonded or coated to the inner column surface.
Non-polar GC columns are best suited for the analysis of non-polar compounds (e.g. alkanes). Equity®-1, Petrocol®, SPB®-1, SPB®-Octyl, and SLB®-5ms are highly recommended products for the separation of non-polar compounds. Polar GC columns are most efficient at separating polar analytes. SPB®-624, OVI-G43, SPB®- 20, Equity®-1701, SPB®-35 SPB®-50, SPB®-225, PAG, SPB®-1000, SLB®-IL60i, and Nukol™ are some of the polar columns that provide the best separation of polar compounds.
Highly polar columns include those that contain stationary phases with a higher percentage of cyanopropyl functional groups. These phases exhibit a variety of interactions amenable to the separation of polarizable compounds. Polarizable compounds are those containing C and H, but with one or more C=C or C≡C bonds. Highly polar capillary columns include SP®-2330, SP®-2331, SP®-2380, SP®-2560, SP®-2340, TCEP, SLB®-IL111i.
Chiral HPLC Column Selection and Method Development Guide
Capillary GC Column Selection and Method Development
