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Catalog No. MN760194.46
Part No. 760194.46
Supplier MACHEREY‑NAGEL
EC HPLC column (analytical), NUCLEODUR 300-5 C4 ec, 5 µm, 125x4.6 mm
Net Price
Special offer
Availability

6 weeks

PROPERTIES
  • Recommended application(s)
    Biological macromolecules, HPLC, Peptides, Proteins
  • Storage temperature
    RT
  • Scope of delivery
    1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
    No
  • Method
    HPLC, analytical
  • Brand
    NUCLEODUR
  • Base material
    NUCLEODUR - high purity silica
  • Phase
    NUCLEODUR 300-5 C4 ec
  • USP listing
    L26
  • Surface chemistry
    Medium density monomeric butyl (C4) wide-pore modification
  • Mode
    Reversed phase (RP)
  • Type
    EC HPLC column (analytical)
  • Hardware
    Stainless steel
  • Column length
    125 mm
  • Column inner diameter
    4.6 mm
  • Particle type
    Fully porous particles (FPP)
  • Particle shape
    Spherical
  • Particle size
    5 µm
  • Eluent in HPLC column
    Acetonitrile – water
  • Endcapped
    Yes
  • Carbon content
    2.5 %
  • pH stability
    1.0–9.0
  • Pore size
    300 Å
  • Specific surface according to BET
    100 m²/g
  • Temperature stability (max)
    60 °C
  • Pressure stability (max)
    450 bar
  • Column volume
    2.06 mL
DESCRIPTION
Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR silica gel and is often used for the separation of biomolecules. Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.
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