MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN732707	CHROMABOND Flash RS 330 SiOH cartridges, 15–40 µm, 330 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 28.0 cm Column inner diameter 59.8 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 330 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 75–255 mL/min pH stability 2.0–8.0 Pressure stability (max) 11 bar Filter element material Polyethylene (PE) Scope of delivery 4x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732708	CHROMABOND Flash RS 800 SiOH cartridges, 15–40 µm, 800 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 38.3 cm Column inner diameter 78.2 mm Column connection Maxi Luer Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 800 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 100–300 mL/min pH stability 2.0–8.0 Pressure stability (max) 7 bar Filter element material Polyethylene (PE) Scope of delivery 2x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732709	CHROMABOND Flash RS 1600 SiOH cartridges, 15–40 µm, 1600 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 43.2 cm Column inner diameter 103.8 mm Column connection Maxi Luer Filling material Irregular, 15–40 µm Hardware Polypropylene (PP) Filling quantity 1600 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15–40 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 200–500 mL/min pH stability 2.0–8.0 Pressure stability (max) 7 bar Filter element material Polyethylene (PE) Scope of delivery 2x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732808	CHROMABOND Flash RS 800 SiOH cartridges, 40–63 µm, 800 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 38.3 cm Column inner diameter 78.2 mm Column connection Maxi Luer Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 800 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 140–240 mL/min pH stability 2.0–8.0 Pressure stability (max) 7 bar Filter element material Polyethylene (PE) Scope of delivery 2x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732809	CHROMABOND Flash RS 1600 SiOH cartridges, 40–63 µm,1600 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 43.2 cm Column inner diameter 103.8 mm Column connection Maxi Luer Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 1600 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 190–250 mL/min pH stability 2.0–8.0 Pressure stability (max) 7 bar Filter element material Polyethylene (PE) Scope of delivery 2x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732850	CHROMABOND Flash RS 3000 SiOH cartridges, 40–63 µm, 3000 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 51.0 cm Column inner diameter 127.5 mm Column connection Maxi Luer Filling material Irregular, 40–63 µm Hardware Polypropylene (PP) Filling quantity 3000 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 40–63 µm Pore size 60 Å Particle shape Irregular Specific surface according to BET 500 m²/g Removable closure Non-removable Typical flow rate 240-390 mL/min pH stability 2.0–8.0 Pressure stability (max) 7 bar Filter element material Polyethylene (PE) Scope of delivery 1x CHROMABOND Flash RS cartridge Storage temperature RT
	MN732460	CHROMABOND Flash RS 4 SPHERE SiOH cartridges, spherical, 25 µm, 4 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 10.6 cm Column inner diameter 12.4 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 25 µm Hardware Polypropylene (PP) Filling quantity 4 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 25 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 10–30 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 20x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732461	CHROMABOND Flash RS 15 SPHERE SiOH cartridges, spherical, 25 µm, 12 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 12.5 cm Column inner diameter 21.2 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 25 µm Hardware Polypropylene (PP) Filling quantity 15 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 25 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 20–45 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 20x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732462	CHROMABOND Flash RS 25 SPHERE SiOH cartridges, spherical, 25 µm, 25 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 17.3 cm Column inner diameter 21.2 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 25 µm Hardware Polypropylene (PP) Filling quantity 25 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 25 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 20–45 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 15x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732463	CHROMABOND Flash RS 40 SPHERE SiOH cartridges, spherical, 25 µm, 40 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 17.6 cm Column inner diameter 26.7 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 25 µm Hardware Polypropylene (PP) Filling quantity 40 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 25 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 25–55 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 15x CHROMABOND Flash RS cartridges Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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