MACHEREY-NAGEL - Sample Preparation - Chromatography - Lab supplies

About 30 years ago MACHEREY‑NAGEL designed and introduced CHROMABOND SPE cartridges containing silica-based adsorbents. SPE is a form of digital (step-wise) chromatography designed to extract, partition, and / or adsorb one or more components from a liquid phase (sample) onto a stationary phase (adsorbent or resin). An adsorbed substance can be removed from the adsorbent by stepwise increase of elution strength of the eluent (step gradient technique). SPE extends a chromatographic system’s lifetime, improves qualitative and quantitative analysis, and the demand placed on an analytical instrument is considerably lessened.

Catalog No.		Product	Net Price
	MN732464	CHROMABOND Flash RS 80 SPHERE SiOH cartridges, spherical, 25 µm, 80 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 24.9 cm Column inner diameter 30.9 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 25 µm Hardware Polypropylene (PP) Filling quantity 80 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 25 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 35–75 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 12x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732465	CHROMABOND Flash RS 120 SPHERE SiOH cartridges, spherical, 25 µm, 120 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 26.2 cm Column inner diameter 37.2 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 25 µm Hardware Polypropylene (PP) Filling quantity 120 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 25 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 40–85 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 10x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732466	CHROMABOND Flash RS 200 SPHERE SiOH cartridges, spherical, 25 µm, 220 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 21.6 cm Column inner diameter 59.4 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 25 µm Hardware Polypropylene (PP) Filling quantity 220 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 25 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 55–110 mL/min pH stability 2.0–8.0 Pressure stability (max) 11 bar Filter element material Polyethylene (PE) Scope of delivery 6x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732467	CHROMABOND Flash RS 330 SPHERE SiOH cartridges, spherical, 25 µm, 330 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 28.0 cm Column inner diameter 59.8 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 25 µm Hardware Polypropylene (PP) Filling quantity 330 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 25 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 55–110 mL/min pH stability 2.0–8.0 Pressure stability (max) 11 bar Filter element material Polyethylene (PE) Scope of delivery 4x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732760	CHROMABOND Flash RS 4 SPHERE SiOH cartridges, spherical, 15 µm, 4 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 10.6 cm Column inner diameter 12.4 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 15 µm Hardware Polypropylene (PP) Filling quantity 4 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 10-20 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 20x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732761	CHROMABOND Flash RS 15 SPHERE SiOH cartridges, spherical,15 µm, 12 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 12.5 cm Column inner diameter 21.2 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 15 µm Hardware Polypropylene (PP) Filling quantity 15 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 20–45 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 20x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732762	CHROMABOND Flash RS 25 SPHERE SiOH cartridges, spherical, 15 µm, 25 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 17.3 cm Column inner diameter 21.2 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 15 µm Hardware Polypropylene (PP) Filling quantity 25 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 20–45 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 15x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732763	CHROMABOND Flash RS 40 SPHERE SiOH cartridges, spherical, 15 µm, 40 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 17.6 cm Column inner diameter 26.7 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 15 µm Hardware Polypropylene (PP) Filling quantity 40 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 25–55 mL/min pH stability 2.0–8.0 Pressure stability (max) 21 bar Filter element material Polyethylene (PE) Scope of delivery 15x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732764	CHROMABOND Flash RS 80 SPHERE SiOH cartridges, spherical, 15 µm, 80 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 24.9 cm Column inner diameter 30.9 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 15 µm Hardware Polypropylene (PP) Filling quantity 80 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 35–75 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 12x CHROMABOND Flash RS cartridges Storage temperature RT
	MN732765	CHROMABOND Flash RS 120 SPHERE SiOH cartridges, spherical,15 µm,120 g	Special offer	Add to Cart
Hazardous material No Method Flash chromatography Brand CHROMABOND Phase SiOH Mode Normal phase (NP) Base material Silica gel Surface chemistry Unmodified silica gel (SiOH) Endcapped No Column type Flash RS Column length 26.2 cm Column inner diameter 37.2 mm Column connection Female Luer lock inlet and male Luer tip outlet Filling material Spherical, 15 µm Hardware Polypropylene (PP) Filling quantity 120 g Recommended application(s) Normal phase Flash chromatography applications (efficient liquid column chromatographic method for preparative applications in the gram range) Particle type Fully porous particles (FPP) Particle size 15 µm Pore size 50 Å Particle shape Spherical Specific surface according to BET 700 m²/g Removable closure Non-removable Typical flow rate 40–85 mL/min pH stability 2.0–8.0 Pressure stability (max) 14 bar Filter element material Polyethylene (PE) Scope of delivery 10x CHROMABOND Flash RS cartridges Storage temperature RT

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Overview

Main steps of the SPE procedure

1) Conditioning

Conditioning of the adsorbent is necessary in order to ensure reproducible interaction with the analyte. Conditioning, also called solvation, results in a wetting of the adsorbent and thus produces an environment, which is suitable for adsorption of the analyte. Nonpolar adsorbents are usually conditioned with 2–3 column volumes of a solvent, which is miscible with water (methanol, THF, 2-propanol etc.), followed by the solvent in which the analyte is dissolved (pure matrix, e.g., water, buffer). Polar adsorbents are conditioned with nonpolar solvents. After the conditioning step the adsorbent bed must not run dry, because otherwise solvation is destroyed (deconditioning).

2) Sample application (adsorption)

Sample application can be performed with positive or negative pressure with a flow rate of ~3 mL/min. Sample volumes vary from a few mL up to liters.

3) Washing

Washing of the adsorbent is usually achieved with a special wash solution; however, in some cases it may not be necessary. If the polarity difference between wash solution and eluent is very large, or if both are not miscible, drying of the adsorbent bed after washing is recommended to improve elution and recovery.

4) Elution

Elution with a suitable eluent should not be too fast. The elution speed depends on the column or cartridge dimension and the quantity of adsorbent (about 1 mL/min).

Since analytes can either be adsorbed on the SPE packing material or directly flown through while the interfering substances are retained, two general separation procedures are possible – both cases are shown in the figure below.

Resources

Instructions CHROMABOND SPE vacuum manifolds for multi 96 monoblocks

Brochure-CHROMABOND-QuEChERS-EN

User manual CHROMABOND SPE vacuum manifolds

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