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Aqueous extraction contains <0.25 EU/mL as determined by the Limulus Amebocyte Lysate (LAL) clot test technique
Catalogue Number
MC0HC10FS1
Chemistry
Cellulose (CL)Inorganic Filter Aid
Device Configuration
Pod
Device Material
Glass fiber reinforced polypropylene
Filter Grade
C0HC
Filtration Area
1.1 m²
Gravimetric Extractables
After 24 hour static soak in pure water (Type 1 DI Water) post autoclave (1 cycle of 60 minutes at 123 °C) and pure water flush of 100 L/m2 of media surface area: 39 – 69 mg/m2
Height
32 cm (12.5 in.)
Length
62 cm (24.2 in.)
Material Size
1
Maximum Differential Pressure, bar (psid)
Forward: 2.1 bar (30 psid) @ 25 °C; 1 bar (15 psid) @ 80 °C. Reverse: 2.1 bar (30 psid) @ 25 °C
Post autoclave (1 cycle of 30 minutes at 123 °C) and pure water flush of 100 L/m2 (10 L/ft2) of media surface area.NVR: 1.50 – 2.02 mg/m2TOC: 416– 593 ppb
Material Size
2
Maximum Differential Pressure, bar (psid)
Forward: 2.07 bar (30 psid) @ 25 °C; 1.03 bar (15 psid) @ 80 °C; 0.21 bar (3 psid) @ 123 °C. Reverse: 0.03 bar (0.5 psid)
Maximum Inlet Pressure, bar (psi)
2.1 bar (30 psi) @ 25 °C; 1 bar (15 psi) @ 80 °C; 0.2 bar (3 psi) @ 123 °C
Oxidizable Substances
The effluent of an autoclaved (1 cycle of 30 minutes @ 123 °C) stacked device is negative after a WFI flush of 10 liters per ft2 (100 L/m2) of media surface area
Sanitization
May be hot water sanitized for 20 cycles using purified water @ 80 °C for 30 minutes
Seal Material
Silicone (SI)
Sterilization
May be steam sterilized for 3 steam-in-place (SIP) cycles of 30 minutes @ 123 °C
Post autoclave (1 cycle of 30 minutes at 123 °C) and pure water flush of 100 L/m2 (10 L/ft2) of media surface area.NVR: 1.50 – 2.02 mg/m2TOC: 416– 593 ppb
Material Size
2
Maximum Differential Pressure, bar (psid)
Forward: 2.07 bar (30 psid) @ 25 °C; 1.03 bar (15 psid) @ 80 °C; 0.21 bar (3 psid) @ 123 °C. Reverse: 0.03 bar (0.5 psid)
Maximum Inlet Pressure, bar (psi)
2.1 bar (30 psi) @ 25 °C; 1 bar (15 psi) @ 80 °C; 0.2 bar (3 psi) @ 123 °C
Oxidizable Substances
The effluent of an autoclaved (1 cycle of 30 minutes @ 123 °C) stacked device is negative after a WFI flush of 10 liters per ft2 (100 L/m2) of media surface area
Sanitization
May be hot water sanitized for 20 cycles using purified water @ 80 °C for 30 minutes
Seal Material
Silicone (SI)
Sterilization
May be steam sterilized for 3 steam-in-place (SIP) cycles of 30 minutes @ 123 °C
Forward: 2.07 bar (30 psid) @ 25 °C; 1.03 bar (15 psid) @ 80 °C; 0.21 bar (3 psid) @ 123 °C. Reverse: 0.03 bar (0.5 psid)
Oxidizable Substances
The effluent of an autoclaved (1 cycle of 30 minutes @ 123 °C) stacked device is negative after a WFI flush of 10 liters per ft2 (100 L/m2) of media surface area
Sanitization
May be hot water sanitized for 20 cycles using purified water @ 80 °C for 30 minutes
Seal Material
Silicone (SI)
Sterilization
May be steam sterilized for 3 steam-in-place (SIP) cycles of 30 minutes @ 123 °C
Aqueous extraction contains <0.25 EU/mL as determined by the Limulus Amebocyte Lysate (LAL) clot test technique
Catalogue Number
MC0HC10FS27EU
Chemistry
Cellulose (CL)Inorganic Filter Aid
Device Configuration
multilayer Pod
Device Material
Glass fiber reinforced polypropylene
Filter Grade
C0HC
Filtration Area
1.1 m²
Gravimetric Extractables
After 24 hour static soak in pure water (Type 1 DI Water) post autoclave (1 cycle of 60 minutes at 123 °C) and pure water flush of 100 L/m2 of media surface area: 39 – 69 mg/m2
Height
32 cm (12.5 in.)
Length
62 cm (24.2 in.)
Material Size
1
Maximum Differential Pressure, bar (psid)
Forward: 2.1 bar (30 psid) @ 25 °C; 1 bar (15 psid) @ 80 °C. Reverse: 2.1 bar (30 psid) @ 25 °C
Aqueous extraction contains <0.25 EU/mL as determined by the Limulus Amebocyte Lysate (LAL) clot test technique
Catalogue Number
MC0HC10FS27NA
Chemistry
Cellulose (CL)Inorganic Filter Aid
Device Configuration
multilayer Pod
Device Material
Glass fiber reinforced polypropylene
Filter Grade
C0HC
Filtration Area
1.1 m²
Gravimetric Extractables
After 24 hour static soak in pure water (Type 1 DI Water) post autoclave (1 cycle of 60 minutes at 123 °C) and pure water flush of 100 L/m2 of media surface area: 39 – 69 mg/m2
Height
32 cm (12.5 in.)
Length
62 cm (24.2 in.)
Material Size
1
Maximum Differential Pressure, bar (psid)
Forward: 2.1 bar (30 psid) @ 25 °C; 1 bar (15 psid) @ 80 °C. Reverse: 2.1 bar (30 psid) @ 25 °C
Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product. Minimal hands-on time 10-minute spin Fully-functional DNA PerformanceTypical DNA Recoveries from Agarose Gels DNA Size (bp) Percent DNA Recoveries 100 78 700 71 1000 77 2027 47 4361 35 9416 32 23130 29Protocol1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
After 24 hour static soak in pure water (Type 1 DI Water) post autoclave (1 cycle of 60 minutes at 123 °C) and pure water flush of 100 L/m2 of media surface area: 39–69 mg/m2
Height
14 cm (5.3 in.)
Length
22 cm (8.5 in.)
Material Size
1
Maximum Differential Pressure, bar (psid)
Forward: 2.1 bar (30 psid) @ 25 °C; 1 bar (15 psid) @ 80 °C. Reverse: 2.1 bar (30 psid) @ 25 °C
After 24 hour static soak in pure water (Type 1 DI Water) post autoclave (1 cycle of 60 minutes at 123 °C) and pure water flush of 100 L/m2 of media surface area: 39–69 mg/m2
Height
14 cm (5.3 in.)
Length
22 cm (8.5 in.)
Material Size
1
Maximum Differential Pressure, bar (psid)
Forward: 2.1 bar (30 psid) @ 25 °C; 1 bar (15 psid) @ 80 °C. Reverse: 2.1 bar (30 psid) @ 25 °C
Trust Merck membrane filtration devices for the collection and preparation of samples, mobile phases, and buffers to obtain the highest quality results from downstream analysis.
Whether you need to collect, separate, or remove contaminants from air or fluid samples prior to downstream analysis, our broad selection of syringe filters, membranes, filter holders and related filtration devices ensures that we have the right solution for your application.
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