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Catalog No. CYT17009010
Part No. 17009010
Supplier Cytiva
Sephadex LH-20, 25 g
Net Price
163
Availability

4 weeks

PROPERTIES
  • Pack size
    100 g
  • Autoclavable
    20 min at 121°C
  • Chemical stability
    Stable in most aqueous and organic eluent systems.
  • Application
    For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
  • Storage
    4 to 30°C
  • Matrix
    Hydroxypropylated, cross-linked dextran
  • Pressure/Flow Specification
    25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
  • pH stability, CIP
    2–13
  • pH stability, operational
    2–13
  • Avoid Using
    Not stable below pH 2 nor to strong oxidising agents
  • Estimated shelf life from manufacture date
    8 years
  • Flow velocity
    < 60 cm/hour
DESCRIPTION
Sephadex LH-20 resin is used for preparative purification of natural products such as steroids, terpenoids, lipids, and low molecular weight peptides. Broad stability in most aqueous mixtures and polar organic solvents. Powerful chromatographic selectivity from the matrix’s dual hydrophilic/lipophilic nature. High chemical and physical robustness. High reproducibility from batch to batch. Sephadex LH-20 is a resin specifically designed for molecular sizing of natural products in organic solvents. Sephadex LH-20 resin for organic solvent useThis chromatography resin is made from cross-linked Sephadex G-25 dextran beads, hydroxypropylated to yield both hydrophilic and lipophilic characteristics. This resin is useful for molecular species preparation at different scales. It can be used during purification, prior to high performance ion exchange or reversed phase chromatography (RPC), or as a final polishing step during diastereomer preparation.Successful use in published researchApplications of Sephadex LH-20 resin include lipid fractionation and purifying complex organic substances, such as traditional Chinese medicine (TCM), for research use. Examples of successful research use include: Antioxidant activity in the stem bark of acacia confusaFree radical scavenging activity in floral corn pollen and pollen found in apis mellifera hives Antioxidant activity in pinus densiflora needles
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