With industry-leading sterile filtration devices based on 60 years of membrane filtration expertise, MilliporeSigma supports conventional and emerging formats for cell culture. The MilliporeSigma Cell Culture Solutions portfolio features over 20,000 high-quality tools to cultivate consistency in your cell culture workflow.
Cell Preparation: Tools that you need to minimize contamination risk and maximize workflow efficiency while preparing your media for cell culture.
Cell Growth: Whatever your cell culture growth needs, we have a plate, insert or vessel that can help you obtain the optimal cell culture results.
Cell Analysis: In addition to our solid base in assay tools and products, we offer new, novel cell culture technologies and cell culture platforms for high content data collection and analysis.
Millicell cell culture insert plates are a patented device designed to support cell growth and differentiation of endothelial and epithelial cell lines, including CaCo-2. The Millicell device is available with a 0.4 µm PCF and a variety of PET membrane pore sizes. The plate is designed for maximum user convenience and incorporates features such as apical assist for easier pipetting and basolateral access. To reduce the risk of monolayer contamination, the membrane plates have feet to elevate the plate above the work surface when disassembled from the feeder tray. Tear drop-shaped receiver wells eliminate air bubbles as plates are assembled.Millicell plate are optimized to grow and sustain high integrity cell monolayers. Cells grown on Millicell plate membranes grow better than on plastic because the cells are nourished from both the apical and basolateral sides. Cell growth and function more closely mimic in vivo. Millicell plates are designed for analysis as well as cell growth and can be used manually or with automated cell seeding, feeding and washing systemsWith this complete system, you can grow, feed, and analyze cells in one membrane-bottom plate. The plate can be used with either a single-well or a multi-well feeding tray. At the time of transport analysis, the plate is simply transferred to a multi-well transport tray for analysis. This streamlined design enhances functionality with: - Seed-and-feed systems - Automated liquid handling systems and span pipettes - Basolateral and apical access to cells - Transepithelial electrical resistance (TEER) measurement systems
Millicell cell culture insert plates are a patented device designed to support cell growth and differentiation of endothelial and epithelial cell lines, including CaCo-2. The Millicell device is available with a 0.4 µm PCF and a variety of PET membrane pore sizes. The plate is designed for maximum user convenience and incorporates features such as apical assist for easier pipetting and basolateral access. To reduce the risk of monolayer contamination, the membrane plates have feet to elevate the plate above the work surface when disassembled from the feeder tray. Tear drop-shaped receiver wells eliminate air bubbles as plates are assembled.Millicell plate are optimized to grow and sustain high integrity cell monolayers. Cells grown on Millicell plate membranes grow better than on plastic because the cells are nourished from both the apical and basolateral sides. Cell growth and function more closely mimic in vivo. Millicell plates are designed for analysis as well as cell growth and can be used manually or with automated cell seeding, feeding and washing systemsWith this complete system, you can grow, feed, and analyze cells in one membrane-bottom plate. The plate can be used with either a single-well or a multi-well feeding tray. At the time of transport analysis, the plate is simply transferred to a multi-well transport tray for analysis. This streamlined design enhances functionality with: - Seed-and-feed systems - Automated liquid handling systems and span pipettes - Basolateral and apical access to cells - Transepithelial electrical resistance (TEER) measurement systems
Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product. Minimal hands-on time 10-minute spin Fully-functional DNA PerformanceTypical DNA Recoveries from Agarose Gels DNA Size (bp) Percent DNA Recoveries 100 78 700 71 1000 77 2027 47 4361 35 9416 32 23130 29Protocol1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
No matter what application you’re working with, Millipore has a plate developed to meet your specific needs. MultiScreen plates have a long history as a reliable tool for the advancement of drug discovery and life science research. HTS Filter Plates (Plate catalogue numbers that start with MS) - MultiScreenHTS plates are specifically developed for high-throughput use with automated work stations. Rigid sidewalls improve handling and ample surfaces are provided for bar code labels. Wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters. Filter plates are available in 96- and 384-well platforms with a broad selection of membranes and plastics. - Millipore’s new MultiScreen HTS+ Hi flow filter plate design lessens non-specific binding and reduces variability in both background and signal intensities specifically for biochemical screening assays.Classic Filter Plates (Plate catalogue numbers that start with MA) - The original multiwell design is valuable for many applications which require immersing the filter in an assay tray. The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users.Accessory – MultiScreenHTS Vacuum Manifold - The MultiScreenHTS Vacuum manifold was designed specifically to work with MultiScreenHTS filter plates. The combination results in optimal filtration characteristics and seamless automation integration.Millicell Assay Plates - In addition to the traditional assay plate format, filter plates are also available with cell culture trays for growing and assaying suspension and adherent cell lines. Cells grown on filter plates differentiate better than those grown on traditional plastic substrates, resulting in more in vivo-like results This filter plate/tray combination can be used for a variety of cellular assays, including, transport, toxicity and screening assays.
No matter what application you’re working with, Millipore has a plate developed to meet your specific needs. MultiScreen plates have a long history as a reliable tool for the advancement of drug discovery and life science research. HTS Filter Plates (Plate catalogue numbers that start with MS) - MultiScreenHTS plates are specifically developed for high-throughput use with automated work stations. Rigid sidewalls improve handling and ample surfaces are provided for bar code labels. Wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters. Filter plates are available in 96- and 384-well platforms with a broad selection of membranes and plastics. - Millipore’s new MultiScreen HTS+ Hi flow filter plate design lessens non-specific binding and reduces variability in both background and signal intensities specifically for biochemical screening assays.Classic Filter Plates (Plate catalogue numbers that start with MA) - The original multiwell design is valuable for many applications which require immersing the filter in an assay tray. The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users.Accessory – MultiScreenHTS Vacuum Manifold - The MultiScreenHTS Vacuum manifold was designed specifically to work with MultiScreenHTS filter plates. The combination results in optimal filtration characteristics and seamless automation integration.Millicell Assay Plates - In addition to the traditional assay plate format, filter plates are also available with cell culture trays for growing and assaying suspension and adherent cell lines. Cells grown on filter plates differentiate better than those grown on traditional plastic substrates, resulting in more in vivo-like results This filter plate/tray combination can be used for a variety of cellular assays, including, transport, toxicity and screening assays.
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